The purpose of the study was to validate the Epsilometer test (E-test) method for antimicrobial susceptibility testing of selected periodontopathic microorganisms. ETEST is a well-established method for antimicrobial susceptibility testing in microbiology laboratories around the world. ETEST consists of a predefined. We describe the applicability of the E test (AB Biodisk Solna, Sweden), a new method for determining minimum inhibitory concentrations of antimicrobial agents.

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Therefore, the E test was found to be a reliable alternative method for the de-termination of MICs of these two fastidious bacterial strains. Retrieved from ” https: Current instruments utilize either the principle of turbidity detection of bacterial growth in a epsiometer medium or detection of hydrolysis of a fluorogenic substrate incorporated in a special liquid medium Accessed October 24, Overview Specs Downloads Publications Overview Quantitative MIC data is a prerequisite for the management of critical infections, including sepsis, especially among critical care patients.

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E Test: a novel technique for antimicrobial susceptibility testing

The correlation between zone sizes and MIC values was then assessed using regression analysis and regression lines were used for extrapolating zone interpretive limits that corresponded to the MIC breakpoint values that defined susceptible, intermediate and resistant categorical results. Evaluation of the ETEST for antimicrobial susceptibility testing of Pseudomonas aeruginosa isolates from patients with long-term bladder catheterization. A positive point of view. The authors are grateful to Dr.

The gradient range is equivalent to 15 log2 dilutions by a conventional reference MIC procedure After 48 hours incubation a drop-shaped inhibition zone intersects the graded test strip at the inhibitory concentration IC of the antibiotic.

The purpose of the study was to validate the Epsilometer test E-test method for antimicrobial susceptibility testing of selected periodontopathic microorganisms using the agar dilution method as a standard. This type of test is most commonly used in healthcare settings to help guiding physicians in treatment of patients by indicating what concentration of antimicrobial would successfully treat an infection.

This page was last edited on 21 Augustat Views Read Edit View history. A report of an international collaborative study. The deficiencies of the disk diffusion test are its non-quantitative interpretation and its inapplicability to many fastidious organisms and anaerobes.


Etest incubation and reading times have been determined based on the intrinsic growth characteristics of the organism, and the specific incubation conditions. After the required incubation period, and only when an even lawn of growth is distinctly visible, the MIC value can be read where the edge of the inhibition ellipse intersects the side of the strip.

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Dilution testing by agar method is also standardized method and a reliable susceptibility testing technique that may be used as a reference for evaluating the accuracy of other testing systems. Etest applications include many groups of fastidious organisms, fungi yeast and mould and mycobacteria as well as detecting various mechanisms of resistance and MIC testing of key antibiotics with critical specimens e. Clinical Product Line Industrial Microbiology.

All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License. In order to determine an MIC with the Epsilometeg test, the surface of an agar plate is swab inoculated with an adjusted bacterial suspension in the same manner as a disk diffusion test.

E-test: a new technique for antimicrobial susceptibility testing for periodontal microorganisms.

Diagn Microbiol Infect Dis, However, the results of such measurements are not an absolute value because they are influenced sometimes markedly, by the test conditions used. The use of microdilution trays prepared in house allows simultaneous testing of several antimicrobial agents against individual organisms epsioometer also provides a reliable standardized reference method for susceptibility testing.

The E test then uses the principle of agar diffusion to perform quantitative testing 4. This report is based on the literature review and on our own experience using the E test for susceptibility testing of the Xanthomonas maltophilia, Streptococcus pneumoniae and Streptococcus viridans group against eight different drugs. The major disadvantages of the agar dilution are the time-consuming and the labor-intensive tasks of preparing the plates and inocula, especially as the number of different antimicrobial agents to be tested against each isolate increases.

Simplex C76, Nema C88, and Retro C80 are easy to use, reducing operator fatigue, saving time and improving the quality of results by increasing reproducibility. National Committee for Clinical Laboratory Standards.


It has shown to be particularly useful for testing fastidious and unusual bacteria that can not be easily tested by more traditional methods, e. It can also be used in a macromethod format to optimize resistance detection.

The inhibition zone sizes from epsliometer test results were compared to Minimum Inhibitory Concentration MIC values based on the reference agar dilution procedure. The E test would be particularly useful in cases for which an MIC of penicillin is required for suspected penicillin-resistant pneumococci, for determining the level of oxacillin resistance in staphylococci, and for measuring the MICs of ampicillin and vancomycin for Enterococcus faecium isolates from severe infections.

Differences in each factor such as inoculum time, may all affect the amount of antimicrobial agent required to inhibit the organism in vitro 7. Etest products for more than antimicrobial agents, including antibiotics, antifungal agents and antimycobacterial agents are available.

E-test: a new technique for antimicrobial susceptibility testing for periodontal microorganisms.

Skip to main content. Additionally, the simultaneous testing of several isolates is possible usually 20 to 37 and microbial contamination or heterogeneity is more readily detected than broth methods.

Plenty of people use the test but very very very few know what 3 stands for. Can we afford to do anaerobic cultures and identification?

Comparison of agar diffusion methodologies for antimicrobial susceptibility testing of Pseudomonas aeruginosa isolates from cystic fibrosis patients. E test strip is a non-porous plastic strip immobilized with predefined continuous and stable gradient of 15 antibiotic concentrations on one side and printed with an MIC scale on the other side of nylon strips.

The convenience afforded by the availability of dilution susceptibility testing in broth microdilution trays has led the widespread use of microdilution methods in the United States and other developed countries.